FeadBead Plate / FeadBead Disc

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Timm Keil

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+49 (0) 241 80 28154

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High throughput feeding system for primary screening processes (Fed-Batch Microplates)

Due to the change of raw materials, common chemical processes will prospectively be replaced or supplemented by biotechnological ways of production. This biotechnological ways of production are based on the cultivation of mostly genetically modified microorganisms or various animal cells.

The development of a biotechnological processes starts with the search for suitable production strains and conditions. Therefore thousands of strains are cultivated and examined for their capability of product formation. This part of the development is called primary screening and designed to be a high throughput procedure. Until today the microorganisms are cultivated in batch mode during primary screening. For this purpose the applied nutrient solution shows a high concentration of substrate and buffer, which covers the demands of the organism over the whole period of cultivation. However many microorganisms show a reduced or even no product formation under this circumstances. Only at the very end of the cultivation when concentrations are low, product formation starts. Strains selected in batch screening conditions will show high productivity when substrate is available in excess, osmotic pressure is high and pH-values are suboptimal. Nevertheless these conditions are far away from the conditions applied in production scale where there are feeding systems (Fed-Batch process) and pH-regulation. This is why strains selected in batch mode during primary screening mostly disappoint in production conditions.

The major target of this project is the further development of micro plates with integrated polymeric-based feeding-systems. In these plates substrates and possible other substances can be released in defined rates and with high reproducibility. In these Feed Plates fed-batch cultivations can be performed without any additional effort and avoid the mentioned problems with batch mode in primary screening.

Literature
Jeude M., Dittirch B., Niederschulte H., Anderlei T., Knocke Ch., Klee D., Büchs J., Fed-batch mode in shake flasks by slow-release technique, Biotechnol. Bioengng. 95(3), 433-445,2006.